《植物生理学报》 2010, 46(11): 1151-1154

节瓜茎尖多胺含量和比值与花性别分化的关系

宋世威, 刘厚诚, 陈日远*

华南农业大学园艺学院, 广州510642

通信作者：陈日远；E-mail: rychen@scau.edu.cn；Tel: 020-38294595

摘　要：

以4 个不同基因型的节瓜为材料, 通过两个发育时期(10、19 片叶展平)茎尖取样, 研究了多胺(PA)含量和比值与植株 花性别分化的关系。结果表明, 节瓜茎尖4 种多胺含量差异显著, 两个取样时期都是亚精胺(Spd)>腐胺(Put)>尸胺(Cad)>精 胺(Spm)。10 片叶展平时期多胺含量与节瓜花性别分化之间没有明确的相关性; 19 片叶展平时期, 节瓜茎尖 Put、Spd 和多 胺总量与植株雌花分化比例呈极显著的正相关, 而Cad则与雌花分化比例呈极显著的负相关。在两个取样时期, 复合指标 Spd/PA 都与植株雌花分化比例呈显著的正相关, 而(Put+Cad)/(Spd+Spm)均与之呈显著的负相关, 可以较好地预测节瓜的 花性别分化状况

关键词：节瓜; 性别分化; 多胺; 相关分析

收稿：2010-07-26   修定：2010-09-07

资助：国家科技支撑计划项目(2008BADA6B07)、科技部星火计划项目(2008GA781001)和广东高校科技成果转化重大项目(cgzhzd0809)

The Relationship between Polyamines Content and Their Ratios in Stem Buds of Chieh-qua and Flower Sex Differentiation

SONG Shi-Wei, LIU Hou-Cheng, CHEN Ri-Yuan*

College of Horticulture, South China Agricultural University, Guangzhou 510642, China

Corresponding author: CHEN Ri-Yuan; E-mail: rychen@scau.edu.cn; Tel: 020-38294595

Abstract:

The relationship between polyamines (PAs) content and their ratios in stem buds and flower sex differentiation in four Chieh-qua (Benincasa hispida Cogn. var. chiehqua How.) cultivars were studied, through sampling of stem buds at two leave development stages (10 and 19 expanded leaves). The results showed that the content of four PAs in stem buds were remarkably different, and the relative content followed the same sequence spermidine (Spd)>putrescine (Put)>cadaverine (Cad)>spermine (Spm) both at two sample stages. The content of single PA had no obvious correlation with differentiation proportion of female flowers at 10 leaves expanded stage. At 19 leaves expanded stage, the content of Put, Spd and total PAs had significantly positive correlation with differentiation proportion of female flowers, while Cad content showed significantly negative correlation with differentiation proportion of female flowers. Both at two sample stages, Spd/PAs was positively correlated with differentiation proportion of female flowers, and (Put+Cad)/(Spd+Spm) was inversely correlated with it remarkably. Thus multiple parameters above could value and forecast flower sex differentiation of Chiehqua.

Key words: Chieh-qua; sex differentiation; polyamine; correlation analysis